Flp-in t-rex core kit manual

Flp-In T-REx Core Kit from Thermo Fisher Scientific. Product Specs; Item Flp-In T-REx Core Kit; Company Thermo Fisher Scientific; Catalog Number K; This product is no longer available on Biocompare. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. Flp In T Rex Core Kit Manual, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. The Flp-In™ System protocol is supplied with the kits listed below. The Core System includes vectors and primers for sequencing. The Complete System includes the Core System plus selection agents. See below for a detailed description of the contents of each Flp-In™ System. Shipping/Storage The Flp-In™ Core System is shipped at room.

For more information about the Flp-In ™ T-REx System, the pOG44 plasmid, and generation of the Flp-In ™ T-REx host cell line, refer to the Flp-In™ T-REx™ Core Kit manual. The Flp-In ™ T-REx Core Kit manual is supplied with the Flp-In™ T-REx™ Core Kit, but is also available for downloading from our Web site (www.doorway.ru) or by. The method to establish the cell clones was essentially done as described in the user's manual of the Flp-In T-REx Core Kit (Invitrogen Catalog no. K) using transfection with lipofectamine (Invitrogen). pFRT/lacZeo2 was linearized with ScaI and Zeocin™ selection was done at μg/ml. pcDNA6/TR was linearized with SapI and blastidin. The HEK cell lines carrying the inducible transgenes were generated essentially as described in the Flp-In™ T-REx™ Core Kit Manual using the cell clone Flp-In™ T-REx™ from Invitrogen. Co-transfection of the Flp expression vector pCSFLPe [28] together with the pcDNA5/FRT/TO vector containing the gene of interest was done with.

The Flp-In ™ T-REx Core Kit manual is supplied with the Flp-In™ T-REx™ Core Kit, but is also available for downloading from our Web site (www.doorway.ru) or by contacting Technical Service (see page 27). The Flp-In™ T-REx™ Cell Line contains pFRT⁄lacZeo and pcDNA™6⁄TR (from the T-REx™ System) stably integrated. Co-transfection of the Flp-In™ Cell Lines with a Flp-In™ expression vector and the Flp recombinase vector, pOG44, results in targeted integration of the expression vector to the same locus in every cell, ensuring homogeneous levels of gene expression. The Flp-In™ T-REx™ System permits polyclonal selection of stable expression cell lines. Generating Flp-In™ T-REx™ Host Cell Lines The Flp-In™ T-REx™ System streamlines the generation of stable, inducible mammalian expression cell lines by taking advantage of a Saccharomyces cerevisiae-derived DNA recombination system.